TROD: T7 RNAi Oligo Designer

Designer siRNAs to overcome the challenges from the RNAi pathway

Small interfering RNA (siRNA)-based technology is playing a pivotal role in understanding gene function. Huge siRNA libraries coupled to high-content screening are being applied to decipher molecular circuitries, as well as to identify novel therapeutic targets. Further, the technology is finding its way towards therapeutic applications. In the midst of all this excitement, the siRNA technology...

Amplification of mRNA populations using aRNA generated from immobilized oligo(dT)-T7 primed cDNA.

products were examined by Southern hybridization with internal oligonucleotide probes and by sequence analysis (not shown). In the study reported here, the ability of the reverse transcriptase enzyme to transcribe full-length cDNA from RNA was followed by a Long PCR reaction to amplify the entire enteroviral coding region together with the 5' and 3' nontranslated regions. The method was tested ...

Removal of double-stranded contaminants from RNA transcripts: synthesis of adenovirus VA RNAI from a T7 vector.

Bacteriophage RNA polymerases are widely used to synthesize defined RNAs on a large scale in vitro. Unfortunately, the RNA product contains a small proportion of contaminating RNAs, including complementary species, which can lead to errors of interpretation. We cloned the gene encoding Ad2 VA RNAI into a vector containing a T7 RNA polymerase promoter in order to generate large quantities of VA ...

‘Designer’ babies

The remaining hurdle to the creation of babies specifically to save the life of an ailing brother or sister was removed in Britain last month by the fertility regulatory body to the delight of afflicted families and alarm of those who fear the advent of a 'designer' baby age. The decision of the UK's Human Fertilisation and Embryology Authority gives new hope to the parents of a two-year-old bo...

Designer Manual